Mechanisms of Gene Expression


Lab Photo April 2017


We study mechanisms of eukaryotic gene expression, focusing on RNA polymerase II transcription initiation, elongation, and termination, as well as the co-transcriptional processing of mRNA.  We use a wide array of approaches, including classic molecular genetics, genomics, protein biochemistry, mass spectrometry, and single molecule microscopy.  

Current projects include:

  • Coupling of mRNA processing to transcription via phosphorylation of the RNA polymerase II C-terminal domain (CTD)
  • The interplay between transcription and chromatin modifications, especially methylations of histone H3
  • The role of overlapping non-coding RNA transcription in modifying mRNA expression
  • The proteomics of transcription initiation and elongation (in collaboration with Jarrod Marto, DFCI/HMS)
  • Single molecule studies of RNA polymerase II transcription (in collaboration with Jeff Gelles, Brandeis University)



Video: Steve talks about the lab's research on gene expression at the 2014 Cold Spring Harbor Lab meeting on the history of mRNA research.

Steve speaking at CSHL


Video: A movie celebrating many years of Robin's cakes for lab birthdays, graduations, etc.!