Understanding the Mechanisms of Gene Expression

Lab Photo April 2019

 

We study mechanisms of eukaryotic gene expression, focusing on RNA polymerase II transcription initiation, elongation, and termination, as well as the co-transcriptional processing of mRNA.  We use a wide array of approaches, including classic molecular genetics, genomics, protein biochemistry, mass spectrometry, and single molecule microscopy.  

Current projects include:

  • Coupling of mRNA processing to transcription via phosphorylation of the RNA polymerase II C-terminal domain (CTD)
  • The interplay between transcription and chromatin modifications, especially methylations of histone H3
  • The role of overlapping non-coding RNA transcription in modifying mRNA expression
  • The proteomics of transcription initiation and elongation (in collaboration with Jarrod Marto, DFCI/HMS)
  • Single molecule studies of RNA polymerase II transcription (in collaboration with Jeff Gelles, Brandeis University)

 


 

Video: Steve talks about the lab's research on gene expression at the 2014 Cold Spring Harbor Lab meeting on the history of mRNA research.


Steve speaking at CSHL


 

Video: A movie celebrating many years of Robin's cakes for lab birthdays, graduations, etc.!

 

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