The Buratowski lab studies mechanisms of eukaryotic gene expression, focusing on RNA polymerase II transcription initiation, elongation, and termination, as well as the co-transcriptional processing of mRNA. We use a wide array of approaches, including classic molecular genetics, genomics, protein biochemistry, mass spectrometry, and single molecule microscopy.
Current projects include:
Coupling of mRNA processing to transcription via phosphorylation of the RNA polymerase II C-terminal domain (CTD)
The interplay between transcription and chromatin modifications, especially methylations of histone H3
The role of overlapping non-coding RNA transcription in modifying mRNA expression
The proteomics of transcription initiation and elongation (in collaboration with Jarrod Marto, DFCI/HMS)
Single molecule studies of RNA polymerase II transcription (in collaboration with Jeff Gelles, Brandeis University)
SOME LAB MOVIES:
Video: Steve presents the lab's single molecule microscopy studies of transcription at the Fragile Nucleosome online series. January 2022.
Video: Steve talks about the lab's research on gene expression at the 2014 Cold Spring Harbor Lab meeting on the history of mRNA research.