We study mechanisms of eukaryotic gene expression, focusing on RNA polymerase II transcription initiation, elongation, and termination, as well as the co-transcriptional processing of mRNA. We use a wide array of approaches, including classic molecular genetics, genomics, protein biochemistry, mass spectrometry, and single molecule microscopy.
Current projects include:
- Coupling of mRNA processing to transcription via phosphorylation of the RNA polymerase II C-terminal domain (CTD)
- The interplay between transcription and chromatin modifications, especially methylations of histone H3
- The role of overlapping non-coding RNA transcription in modifying mRNA expression
- The proteomics of transcription initiation and elongation (in collaboration with Jarrod Marto, DFCI/HMS)
- Single molecule studies of RNA polymerase II transcription (in collaboration with Jeff Gelles, Brandeis University)
Video: Steve talks about the lab's research on gene expression at the 2014 Cold Spring Harbor Lab meeting on the history of mRNA research.